The nervous system is a complex system made up of a plethora of cell types. Neurological disorders represent a leading cause of death worldwide and are subject to rigorous research. Alzheimer’s is a life-limiting form of dementia that is associated with memory loss and reduced quality of life. Aging is known to play a role in the onset of Alzheimer’s, but the exact cause of onset is not fully understood. Single cell RNA-sequencing has enabled neurobiological research to dive into the level of granularity needed to address the cellular complexity of the CNS and develop insights into neurological diseases.
Join us for this Front Line Genomics webinar that dives into how single cell and spatial technologies are transforming neurobiological research, with insights on how white matter aging drives microglial diversity in Alzheimer’s.
White matter aging drives microglial diversity – Ozgun Gokce, Group Leader, LMU University Hospital Munich, Institute for Stroke and Dementia Research (ISD)
Aging results in gray and white matter degeneration, but the specific microglial responses are unknown. Using single-cell RNA sequencing from white and gray matter separately, we identified white matter-associated microglia (WAMs), which share parts of the disease-associated microglia (DAM) gene signature and are characterized by activation of genes implicated in phagocytic activity and lipid metabolism. WAMs depend on triggering receptor expressed on myeloid cells 2(TREM2) signaling and are aging dependent. In the aged brain, WAMs form independent of apolipoprotein E (APOE), in contrast to mouse models of Alzheimer’s disease, in which microglia with the WAM gene signature are generated prematurely and in an APOE-dependent pathway similar to DAMs. Within the white matter, microglia frequently cluster in nodules, where they are engaged in clearing degenerated myelin. Thus, WAMs may represent a potentially protective response required to clear degenerated myelin accumulating during white matter aging and disease.
Deciphering complex biology in neuroscience with single cell and spatial technologies – Hannes Arnold, Senior Science & Technology Advisor, 10x Genomics
The central nervous system (CNS) is a complex network of diverse cell types with a myriad of functions, communicating via dynamic signaling pathways and synaptic interactions. The study of the CNS requires tools that provide unbiased, high-resolution approaches, ultimately, generating deeper insight into the diverse, complex cellular and signaling networks that control CNS function.
During this webinar, you’ll learn how Chromium Single Cell Gene Expression and Visium Spatial Gene Expression products let you:
- Interrogate the cellular and molecular mechanisms that underlie normal development, neural function, disease, and injury with single cell gene expression
- Investigate the epigenetic mechanisms of gene regulation with single cell epigenomics and discover insights into the regulatory landscape of the CNS genome and transcriptome
- Examine the dynamic nature of gene expression patterns and regional gene expression alterations, and uncover context as to how these may contribute to normal development as well as developmental or neurodegenerative disorders
This webinar will introduce Chromium and Visium solutions for multimodal single cell analysis and spatially resolved transcriptomic analysis of nervous tissues and cerebrospinal fluid. Examples of customer publications and data analysis will be presented.
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This webinar series has been made possible due to the kind support of our partner 10x Genomics.